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Martin Zoltner, Nina Krienitz, Mark C. Field, Susanne Kramer
Comparative proteomics of the two T. brucei PABPs suggests that PABP2 controls bulk mRNA
www.plosntds.org
www.plosntds.org
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565.0583 667.3323 l
h
f*
298.9984 641.3102 m
308.5228 641.3102 l
h
f*
512.674 641.3102 m
522.1984 641.3102 l
h
f*
323.6031 602.3055 m
333.1276 602.3055 l
h
f*
335.3953 602.3055 m
344.863 602.3055 l
h
f*
290.8913 589.3228 m
300.4157 589.3228 l
h
f*
305.5748 589.3228 m
315.0992 589.3228 l
h
f*
334.3181 576.3402 m
343.8425 576.3402 l
h
f*
346.1102 576.3402 m
355.6346 576.3402 l
h
f*
357.8457 576.3402 m
367.3701 576.3402 l
h
f*
555.3071 576.3402 m
564.8315 576.3402 l
h
f*
372.189 550.3181 m
381.7134 550.3181 l
h
f*
268.1575 511.3134 m
277.6819 511.3134 l
h
f*
338.1732 498.3307 m
347.6409 498.3307 l
h
f*
256.0819 485.348 m
265.6063 485.348 l
h
f*
267.8173 485.348 m
277.3417 485.348 l
h
f*
331.0299 459.326 m
340.5543 459.326 l
h
f*
234.8787 420.3213 m
244.4031 420.3213 l
h
f*
203.4142 368.3339 m
212.9386 368.3339 l
h
f*
457.285 368.3339 m
466.8094 368.3339 l
h
f*
457.9654 355.3512 m
467.4331 355.3512 l
h
f*
469.7008 355.3512 m
479.2252 355.3512 l
h
f*
429.8457 329.3291 m
439.3701 329.3291 l
h
f*
441.6378 329.3291 m
451.1622 329.3291 l
h
f*
305.2913 316.3465 m
314.8157 316.3465 l
h
f*
317.0268 316.3465 m
326.5512 316.3465 l
h
f*
231.7039 303.3071 m
241.1717 303.3071 l
h
f*
389.1402 303.3071 m
398.6079 303.3071 l
h
f*
400.8756 303.3071 m
410.4 303.3071 l
h
f*
492.8882 290.3244 m
502.4126 290.3244 l
h
f*
456.2079 264.3024 m
465.7323 264.3024 l
h
f*
467.9433 264.3024 m
477.4677 264.3024 l
h
f*
537.7323 251.3197 m
547.2567 251.3197 l
h
f*
370.6583 238.337 m
380.1827 238.337 l
h
f*
292.7055 225.2976 m
302.1732 225.2976 l
h
f*
0 g
1 j
1 J
0 w
10 0 0 10 200.0125 707.4141 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(described)Tj
4.0535 0 Td
(\(recently)Tj
3.7473 0 Td
(reviewed)Tj
3.7928 0 Td
(in)Tj
1.0375 0 Td
([)Tj
0.83 0.64 0.02 0 k
(31)Tj
0 g
(].)Tj
2.0806 0 Td
(The)Tj
1.7801 0 Td
(best)Tj
1.8085 0 Td
(characterised)Tj
5.5049 0 Td
(complex)Tj
3.6226 0 Td
(comprises)Tj
4.2746 0 Td
(eIF4E4,)Tj
-31.7024 -1.3039 Td
(eIF4G3,)Tj
3.4015 0 Td
(eIF4A1)Tj
3.1464 0 Td
(and)Tj
1.7292 0 Td
(PABP1)Tj
3.0784 0 Td
(in)Tj
1.0374 0 Td
(both)Tj
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2.058 0 Td
[()-3(\r)2()-4()12()18( )6()16()18()4()]TJ
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4.7905 0 Td
(and)Tj
1.7291 0 Td
(trypanosomes)Tj
5.8224 0 Td
([)Tj
0.83 0.64 0.02 0 k
(33)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(37)Tj
0 g
(].)Tj
3.5489 0 Td
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3.8551 0 Td
(of)Tj
1.0148 0 Td
(a)Tj
-35.2117 -1.2982 Td
(direct)Tj
2.5284 0 Td
(physical)Tj
3.4526 0 Td
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4.6091 0 Td
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(eIF4E4)Tj
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(eIF4G3)Tj
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1.0374 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
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0.9695 0 Td
[( )6()16()3()11()]TJ
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2.5682 0 Td
(using)Tj
2.3924 0 Td
(yeast)Tj
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(two)Tj
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1.5534 0 Td
(direct)Tj
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(between)Tj
3.532 0 Td
(LmPABP1)Tj
4.4333 0 Td
(and)Tj
1.7291 0 Td
(eIF4G3)Tj
3.1805 0 Td
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1.7008 0 Td
(not)Tj
1.5704 0 Td
(observed)Tj
3.7984 0 Td
([)Tj
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(35)Tj
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(,)Tj
0.83 0.64 0.02 0 k
(37)Tj
0 g
(].)Tj
-34.0382 -1.2983 Td
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3.3788 0 Td
(LmPABP1)Tj
4.4334 0 Td
(interacted)Tj
4.2349 0 Td
(directly)Tj
3.2315 0 Td
(with)Tj
2.0012 0 Td
(eIF4E4,)Tj
3.2542 0 Td
(mediated)Tj
3.9458 0 Td
(by)Tj
1.1792 0 Td
(the)Tj
1.4683 0 Td
(non-conserved)Tj
6.2192 0 Td
(N-termi-)Tj
-33.3465 -1.3039 Td
(nal)Tj
1.4456 0 Td
(extension)Tj
4.0649 0 Td
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(eIF4E4)Tj
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([)Tj
0.83 0.64 0.02 0 k
(35)Tj
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2.075 0 Td
(an)Tj
1.2018 0 Td
(interaction)Tj
4.6091 0 Td
(critical)Tj
2.9481 0 Td
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1.3889 0 Td
(the)Tj
1.4684 0 Td
(function)Tj
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(of)Tj
1.0148 0 Td
(eIF4E4)Tj
3.033 0 Td
([)Tj
0.83 0.64 0.02 0 k
(38)Tj
0 g
(].)Tj
2.075 0 Td
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1.7858 0 Td
(cur-)Tj
-34.7865 -1.2982 Td
(rent)Tj
1.8595 0 Td
(assumption)Tj
4.8642 0 Td
(that)Tj
1.7745 0 Td
(eIF4E4/eIF4G3/PABP)Tj
8.8157 0 Td
(1)Tj
0.6916 0 Td
(is)Tj
0.8504 0 Td
(the)Tj
1.4683 0 Td
(major)Tj
2.6079 0 Td
(translation)Tj
4.5354 0 Td
(initiation)Tj
3.9231 0 Td
(complex)Tj
3.6227 0 Td
(is)Tj
-35.0133 -1.2983 Td
(predicated)Tj
4.4333 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4683 0 Td
(following:)Tj
4.2066 0 Td
(i\))Tj
0.8277 0 Td
(all)Tj
1.1452 0 Td
(proteins)Tj
3.515 0 Td
(are)Tj
1.4456 0 Td
(of)Tj
1.0148 0 Td
(high)Tj
2.0069 0 Td
(abundance,)Tj
4.8246 0 Td
(ii\))Tj
1.0941 0 Td
(PABP1)Tj
3.0728 0 Td
(has)Tj
1.5477 0 Td
(greater)Tj
3.0047 0 Td
(speci-)Tj
-34.8829 -1.3039 Td
(ficity)Tj
2.2166 0 Td
(for)Tj
1.389 0 Td
(poly\(A\))Tj
3.3052 0 Td
(than)Tj
2.0296 0 Td
(PABP2)Tj
3.0784 0 Td
([)Tj
0.83 0.64 0.02 0 k
(36)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(39)Tj
0 g
(],)Tj
3.2541 0 Td
(iii\))Tj
1.3606 0 Td
(eIF4E4)Tj
3.0331 0 Td
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2.4321 0 Td
(the)Tj
1.4683 0 Td
(type)Tj
1.9163 0 Td
(4)Tj
0.6916 0 Td
(cap)Tj
1.5987 0 Td
(with)Tj
1.9956 0 Td
(the)Tj
1.4683 0 Td
(highest)Tj
3.0898 0 Td
(affinity)Tj
-34.3273 -1.2983 Td
(of)Tj
1.0147 0 Td
(all)Tj
1.1452 0 Td
(eIF4E4)Tj
3.0331 0 Td
(paralogs)Tj
3.5546 0 Td
([)Tj
0.83 0.64 0.02 0 k
(40)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(42)Tj
0 g
(])Tj
3.3222 0 Td
(and)Tj
1.7291 0 Td
(iv\))Tj
1.2813 0 Td
(silencing)Tj
3.77 0 Td
(of)Tj
1.0148 0 Td
(eIF4E4,)Tj
3.2598 0 Td
(eIF4G3)Tj
3.1748 0 Td
(and)Tj
1.7291 0 Td
(PABP1)Tj
3.0785 0 Td
(in)Tj
1.0374 0 Td
(at)Tj
0.9525 0 Td
(least)Tj
1.9842 0 Td
(some)Tj
/F9 1 Tf
-35.0813 -1.2983 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F9 1 Tf
1.0318 0 Td
[(\n)11()-9()4()7(\r)2()]TJ
/F8 1 Tf
2.6192 0 Td
(life)Tj
1.4456 0 Td
(cycle)Tj
2.1883 0 Td
(stages)Tj
2.5625 0 Td
(is)Tj
0.8504 0 Td
(lethal)Tj
2.3925 0 Td
([)Tj
0.83 0.64 0.02 0 k
(33)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(34)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(36)Tj
0 g
(])Tj
4.2065 0 Td
(and)Tj
1.7292 0 Td
(eIF4E4)Tj
3.033 0 Td
(cannot)Tj
2.9821 0 Td
(be)Tj
1.1508 0 Td
(deleted)Tj
3.0898 0 Td
(in)Tj
/F9 1 Tf
1.0318 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F9 1 Tf
0.9751 0 Td
[()12()11()9()16()22()7()12( )]TJ
/F8 1 Tf
3.9004 0 Td
([)Tj
0.83 0.64 0.02 0 k
(38)Tj
0 g
(].)Tj
-33.9928 -1.3039 Td
(At)Tj
1.2075 0 Td
(least)Tj
1.9786 0 Td
(three)Tj
2.2677 0 Td
(additional)Tj
4.2746 0 Td
(translation)Tj
4.5354 0 Td
(initiation)Tj
3.9231 0 Td
(complexes)Tj
4.405 0 Td
(are)Tj
1.4514 0 Td
(known.)Tj
3.2315 0 Td
(The)Tj
1.7801 0 Td
(first)Tj
1.8085 0 Td
(consists)Tj
3.3449 0 Td
(of)Tj
-35.4045 -1.2982 Td
(eIF4E5)Tj
3.033 0 Td
(bound)Tj
2.8346 0 Td
(to)Tj
1.0262 0 Td
(either)Tj
2.5341 0 Td
(eIF4G1)Tj
3.1748 0 Td
(or)Tj
1.0998 0 Td
(eIF4G2)Tj
3.1748 0 Td
([)Tj
0.83 0.64 0.02 0 k
(41)Tj
0 g
(].)Tj
2.0806 0 Td
(Two)Tj
2.0183 0 Td
(further)Tj
3.033 0 Td
(proteins)Tj
3.5093 0 Td
(specifically)Tj
4.5751 0 Td
(interact)Tj
3.2825 0 Td
(with)Tj
-35.3761 -1.2983 Td
(the)Tj
1.4683 0 Td
(eIF4G1)Tj
3.1804 0 Td
(version)Tj
3.1521 0 Td
(of)Tj
1.0148 0 Td
(this)Tj
1.6725 0 Td
(complex:)Tj
3.8494 0 Td
(TbG1-IP)Tj
3.7757 0 Td
(\(Tb927.11.6720\))Tj
6.7521 0 Td
(and)Tj
1.7291 0 Td
(TbG1-IP2)Tj
4.252 0 Td
(\(Tb927.11.350\).)Tj
-30.8464 -1.3039 Td
(TbG1-IP)Tj
3.7757 0 Td
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0.8504 0 Td
(an)Tj
1.2018 0 Td
(mRNA)Tj
3.0841 0 Td
(cap)Tj
1.5931 0 Td
(guanine-N7)Tj
4.9946 0 Td
(methyltransferase,)Tj
7.5344 0 Td
(suggesting)Tj
4.3994 0 Td
(involvement)Tj
5.21 0 Td
(in)Tj
1.0375 0 Td
(nuclear)Tj
-33.681 -1.2983 Td
(mRNA)Tj
3.0784 0 Td
(capping)Tj
3.3958 0 Td
([)Tj
0.83 0.64 0.02 0 k
(43)Tj
0 g
(],)Tj
2.075 0 Td
(but)Tj
1.5534 0 Td
(such)Tj
2.0579 0 Td
(a)Tj
0.6576 0 Td
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0.8447 0 Td
(unlikely,)Tj
3.6566 0 Td
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1.0148 0 Td
(the)Tj
1.4741 0 Td
(protein)Tj
3.1521 0 Td
(is)Tj
0.8447 0 Td
(cytoplasmic)Tj
4.9719 0 Td
(and)Tj
1.7348 0 Td
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-34.1402 -1.2982 Td
(to)Tj
1.0261 0 Td
(starvation)Tj
4.1896 0 Td
(stress)Tj
2.3981 0 Td
(RNA)Tj
2.262 0 Td
(granules)Tj
3.6 0 Td
([)Tj
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(44)Tj
0 g
(],)Tj
2.0749 0 Td
(and)Tj
1.7291 0 Td
(nuclear)Tj
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(cap)Tj
1.5931 0 Td
(methylation)Tj
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(TbCGM1)Tj
4.0875 0 Td
([)Tj
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(45)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(46)Tj
0 g
(].)Tj
3.2541 0 Td
(TbG1-IP2)Tj
4.252 0 Td
(is)Tj
0.8504 0 Td
(an)Tj
1.2019 0 Td
(RNA)Tj
2.262 0 Td
(binding)Tj
3.3505 0 Td
(protein)Tj
3.1521 0 Td
(with)Tj
1.9956 0 Td
(unknown)Tj
4.0819 0 Td
(function.)Tj
3.8551 0 Td
(The)Tj
1.7801 0 Td
(sec-)Tj
-35.3024 -1.3039 Td
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1.8028 0 Td
(complex)Tj
3.6226 0 Td
(consists)Tj
3.3449 0 Td
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1.0148 0 Td
(eIF4G5,)Tj
3.4072 0 Td
(which)Tj
2.6476 0 Td
(specifically)Tj
4.5694 0 Td
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3.651 0 Td
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1.9955 0 Td
(eIF4E6)Tj
3.0331 0 Td
(and)Tj
1.7291 0 Td
(one)Tj
1.7008 0 Td
(further)Tj
3.0274 0 Td
(pro-)Tj
-35.5462 -1.2983 Td
(tein)Tj
1.7574 0 Td
(TbG5-IP)Tj
3.7757 0 Td
(\(Tb927.11.14590\))Tj
7.2283 0 Td
([)Tj
0.83 0.64 0.02 0 k
(42)Tj
0 g
(].)Tj
2.075 0 Td
(Interestingly,)Tj
5.4935 0 Td
(similarly)Tj
3.685 0 Td
(to)Tj
1.0262 0 Td
(TbG1-IP1,)Tj
4.4787 0 Td
(this)Tj
1.6781 0 Td
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3.6056 0 Td
(a)Tj
0.6576 0 Td
(nucleoside)Tj
4.4731 0 Td
(triphosphate)Tj
5.2724 0 Td
(hydrolase)Tj
4.0705 0 Td
(and)Tj
1.7291 0 Td
(a)Tj
0.6577 0 Td
(guanylyltransferase)Tj
7.9086 0 Td
(domain)Tj
3.3278 0 Td
(in)Tj
1.0318 0 Td
(common)Tj
-32.7342 -1.3039 Td
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1.9955 0 Td
(enzymes)Tj
3.6624 0 Td
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3.651 0 Td
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1.0374 0 Td
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1.5931 0 Td
(formation.)Tj
4.4844 0 Td
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1.7858 0 Td
(third)Tj
2.211 0 Td
(complex)Tj
3.6227 0 Td
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3.3448 0 Td
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1.0148 0 Td
(eIF4G4,)Tj
3.4072 0 Td
(eIF4E3)Tj
3.0331 0 Td
(and)Tj
-34.8432 -1.2982 Td
(eIF4A1)Tj
3.1464 0 Td
([)Tj
0.83 0.64 0.02 0 k
(33)Tj
0 g
(].)Tj
2.0749 0 Td
(However,)Tj
4.0762 0 Td
(neither)Tj
3.0841 0 Td
(PABP)Tj
2.6022 0 Td
(was)Tj
1.6951 0 Td
(identified)Tj
4.0592 0 Td
(in)Tj
1.0375 0 Td
(any)Tj
1.6554 0 Td
(of)Tj
1.0148 0 Td
(these)Tj
2.2563 0 Td
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-25.5059 -1.2983 Td
(Several)Tj
3.05 0 Td
(studies)Tj
2.9877 0 Td
(have)Tj
2.058 0 Td
(directly)Tj
3.2371 0 Td
(addressed)Tj
4.1726 0 Td
(function,)Tj
3.8551 0 Td
(substrate)Tj
3.804 0 Td
(specificity)Tj
4.2123 0 Td
(and)Tj
1.7291 0 Td
(localisation)Tj
4.7565 0 Td
(of)Tj
-35.0586 -1.3039 Td
(kinetoplastid)Tj
5.4084 0 Td
(poly\(A\)-binding)Tj
6.7918 0 Td
(proteins.)Tj
3.7417 0 Td
(PABP1)Tj
3.0784 0 Td
(and)Tj
1.7291 0 Td
(PABP2)Tj
3.0784 0 Td
(are)Tj
1.4457 0 Td
(highly)Tj
2.7099 0 Td
(abundant)Tj
4.0478 0 Td
(and)Tj
1.7292 0 Td
(in)Tj
1.0374 0 Td
(excess)Tj
-34.7978 -1.2983 Td
(of)Tj
1.0147 0 Td
(the)Tj
1.4684 0 Td
(total)Tj
2.0012 0 Td
(number)Tj
3.4186 0 Td
(of)Tj
1.0148 0 Td
(mRNA)Tj
3.0841 0 Td
(molecules,)Tj
4.422 0 Td
(at)Tj
0.9524 0 Td
(least)Tj
1.9843 0 Td
(in)Tj
1.0374 0 Td
(the)Tj
1.4684 0 Td
(procyclic)Tj
3.8551 0 Td
(life)Tj
1.4456 0 Td
(cycle)Tj
2.1884 0 Td
(stage)Tj
2.1996 0 Td
(of)Tj
/F9 1 Tf
1.0148 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F9 1 Tf
1.0375 0 Td
[(\n)11()-9()4()7(\r)2()]TJ
/F8 1 Tf
-33.6073 -1.2983 Td
([)Tj
0.83 0.64 0.02 0 k
(36)Tj
0 g
(].)Tj
2.0749 0 Td
(RNAi)Tj
2.5285 0 Td
(in)Tj
/F9 1 Tf
1.0375 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
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2000 Adobe Systems Incorporated. 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