Microbial eukaryotes represent a huge component of the biosphere, with contributions to ecology, food security and the environment. Parasitic diseases caused by protozoa constitute a major global threat. Our work melds several key aspects of protist biology, principally focusing on the parasitic organisms of the trypanosome group, but also extending into environmental organisms, such as the algae Euglena. We are active in comparative genomics and experimental cell biology, using these approaches to understand how eukaryotes have evolved, as well as to develop methodologies for the functional understanding of protist diversity and disease mechanisms. We are committed to open access publishing and rapid, full disclosure of data. Our principle research objectives are the discovery, functional characterisation and validation of novel gene products, drug targets and chemical tools towards neglected diseases to provide therapy, deep insights into protist cell biology and to understand eukaryotic evolution at the molecular level.
Endocytotic systems of trypanosomes: The role of components of the endocytotic pathway in drug sensitivity, virulence in vivo and protein/lipid sorting mechanisms.
Nuclear architecture and function in trypanosomes: Analysis of the trypanosome nuclear pore complex and nuclear envelope, and impact on gene expression and virulence.
Evolutionary biology, eukaryogenesis, eukaryotic microbial diversity: Reconstructing the evolutionary history of intracellular transport pathways and origins of the eukaryotic cell though comparative genomics, genome sequencing and proteomics.
Suramin exposure alters cellular metabolism and mitochondrial energy production in African trypanosomes. Zoltner, M., Campagnaro, G.D., Taleva, G., Burrell, A., Cerone, M., Leung, K-F., Achcar, F., Horn, D., Vaughan, S., Gadelha, C., Zíková, A., Barrett, M.P., deKoning, H.P., and Field, M.C., (2020) Journal of Biological Chemistry 295 8331
Monoallelic activator sequestration by a histone chaperone for heritable allelic exclusion. Faria, J., Glover, L., Hutchinson, S., Boehm, C., Field, M.C., and Horn, D., (2019) Nature Communications 10 3023
The kinetochore and the origin of eukaryotic chromosome segregation. Field, M.C., (2019) Proceedings of the National Academy of Sciences (USA) 116 12596
Pore timing: the evolutionary origins of the nucleus and nuclear pore complex. Field, M.C., and Rout, M., (2019) F1000 Research 8 369
Transcriptome, proteome and draft genome of Euglena gracilis. Ebenezer, T., Zoltner, M., Burrell, A., Nenarokova, A., Novák Vanclová, A.M.G., Prasad, B., Soukal, P., Santana-Molina, C., O’Neill, E., Nankissoor, N.N., Vadakedath, N., Daiker, V., Obado, S., Silva-Pereira, S., Jackson, A.P., Devos D., Lukeš, J., Lebert, M., Vaughan, S., Hampl, V., Carrington, M., Ginger, M.L., Dacks, J.B., Kelly, S., and Field, M.C., (2019) BMC Biology 17 11
Evolutionary origins and specialisations of membrane transport. Dacks, J.B., and Field, M.C. (2018) Current Opinions in Cell Biology 53 70 - 76
Activation and mechanisms of resistance to phenoxyborole class trypanosides. Zhang, N., Zoltner, M., Ka-Fai
Leung, K-F., Scullion, P., Hutchinson, S., del Pino, R.C., Vincent, I., Zhang, Y-K., Freund, Y.R., Alley, M.R.K., Jacobs, R.T., Read, K.D., Barrett, M.P., Horn, D., and Field, M.C., (2018) PLoS Pathogens 14 e1006850
The evolution of organellar coat complexes and organisation of the eukaryotic cell. Rout, M.P., and Field, M.C., (2017) Annual Reviews of Biochemistry 86 637 - 657
Lineage-specific proteins essential for endocytosis in trypanosomes. Manna, P.T., Obado, S., Boehm, C., Gadelha, C., Sali, A., Chait, B.T., Rout, M.P., and Field, M.C., (2017) Journal of Cell Science 130 1379 - 1392
Co-dependence between Trypanosoma nuclear lamina components in nuclear stability and control of gene expression. Maishman, L., Obado, S., Alsford, S., Bart, J.M., Navarro, M., Horn, D., Ratushny, A., Aitchison, J.D., Chait, B.T., Rout, M.P., and Field, M.C., (2016) Nucleic Acids Research 44 10554-10570
Ancient eukaryotic origin and evolutionary plasticity of the nuclear lamina. Koreny, L., and Field, M.C., (2016) Genome Biology and Evolution 8 2663 - 2671
FIELD LABORATORY Cell biology, Evolution, Parasites, Drugs